The flow cytometer Diaries
The flow cytometer Diaries
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Data-enriched characterization of mobile populations. Morphological details from visuals can add on the richness of flow cytometry information. By way of example, the figure displays an or else regular apoptosis assay working with Annexin V and PI, adding cell imaging to characterize cells in Each individual populace to reveal morphologically unique functions. These insights couldn't are actually attained from multiplex staining by yourself.
As samples are acquired about the Attune CytPix Flow Cytometer, the substantial-velocity brightfield camera captures and retailers visuals of detected events in a level nearly 6,000 pictures for each next, depending upon the flow price and impression sizing. For larger overall flexibility, Attune Cytometric Software helps you to alter the impression capture frequency as required and select a specific gate for impression capture, if demanded.
(B) RAW 264.seven macrophages are sometimes annotated inaccurately with regard to particle count due to their complexity and noncircular borders. The volume of singlets available for Evaluation improves as soon as the user trains and employs a more correct product.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing using a higher-speed brightfield digital camera for simultaneous significant throughput flow cytometry and substantial resolution brightfield imaging.
"A picture isn't really really really worth a thousand dots, nevertheless it actually helps you to see images. It is excellent to check out photographs that correlate with staining. One example is, AnnexinV-optimistic cells which can be morphologically unique than wholesome cells, or CD14+ monocytes which are more substantial and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it really is illuminating to find out how many doublets slip by way of conventional singlet gates, and excellent to verify the position of unexpected double-beneficial situations in my Examination."
Pair your Attune flow cytometer with an autosampler for greater workflow effectiveness, and insert robotic integration for optimum throughput.
Our researchers will layout panels for you personally as again and again you may need—totally free, no item obtain wanted.
The Attune NxT cytometer is definitely the reputable workhorse instrument your lab can count on for consistent and trustworthy general performance. The Attune CytPix design features a superior-speed brightfield digital camera and AI-driven image analysis program, with the latest upgrades including equipment Understanding instruments and an intuitive consumer interface, enabling enhanced details precision, accuracy, and speedier insights into sample biology.
Customers can watch event pictures in actual-time to confirm sample top quality, enhance protocols, and document the morphology of each and every mobile populace discovered for analysis. Imaging seize also Positive aspects from acoustic concentrating, which helps to position the cells to ensure a pointy, centered picture is received.
Seem waves allow precise mobile alignment, leading to Fantastic knowledge excellent and ~10X quicker sample flow charges.
We Formerly shown the power of imaging Automobile-T/Ramos mobile interactions. Enable’s take a look at just the populace of greatest curiosity, the double constructive occasions, to learn more. We are able to now use extended morphological parameters (here, circularity and skewness of depth) to further look at the characteristics of those populations and refine gating on these situations, rising details robustness.
Leverage the power of State-of-the-art technological innovation and innovation to speed up your discoveries. Have confidence in while in the Attune Flow Cytometer family to provide the trustworthiness, velocity, and accuracy that set your function aside.
No clean/no lyse leukocyte Investigation with imaging. Cells have been obtained from 24-hour-old blood by dilution in 1 mM EDTA ( Expert UX/UI allows consumers to teach AI products for expanded applications. (A) The “Train Model” feature presents annotation applications and real-time design teaching precision.
At the guts of each and every Attune Flow Cytometer lies our innovative acoustic concentrating technological innovation, which makes use of audio waves to meticulously align cells inside the flow mobile. This revolutionary technique surpasses conventional hydrodynamic solutions, leading to more quickly processing speeds, Fantastic info good quality, and improved sample throughput.
The Attune Xenith cytometer can be a significant-parameter spectral analyzer, providing both spectral unmixing and traditional flow capabilities for in depth and functional mobile analysis.
Agilent Value Promise guarantees you no less than a decade of instrument use from your date you buy. If not, We'll credit rating you Using the residual price of the procedure toward an upgraded product.
Here, we exhibit that by gating tactics making use of picture-centered quantitative parameters can distinguish interacting cells from coincident events to a lot more precisely assess interacting more info cells.